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  • Featured Product
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NSMCE2 Polyclonal antibody

NSMCE2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13627-1-AP

Print datasheet

Synonyms

C8orf36, E3 SUMO protein ligase NSE2, hMMS21, MMS21, MMS21 homolog, Non SMC element 2 homolog, NSE2, NSMCE2

验证数据展示

  • WB analysis of HeLa using 13627-1-AP

    WB analysis of HeLa using 13627-1-AP

    WB result of NSMCE2 antibody (13627-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSMCE2 transfected HeLa cells.

  • WB analysis using 13627-1-AP

    WB analysis using 13627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13627-1-AP (NSMCE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 13627-1-AP

    IHC staining of mouse testis using 13627-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13627-1-AP (NSMCE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, U2OS cells
Positive IHC detected inmouse testis tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13627-1-AP targets NSMCE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NSMCE2 fusion protein Ag4545 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name non-SMC element 2, MMS21 homolog (S. cerevisiae)
Synonyms C8orf36, E3 SUMO protein ligase NSE2, hMMS21, MMS21, MMS21 homolog, Non SMC element 2 homolog, NSE2, NSMCE2
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC032797
Gene Symbol NSMCE2
Gene ID (NCBI) 286053
RRIDAB_10637854
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NSMCE2 antibody 13627-1-APDownload protocol
IHC protocol for NSMCE2 antibody 13627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.

Authors - Stephanie Pei Tung Yiu
View Article