• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85188-4-RR (same clone as 85188-4-PBS)

    WB analysis using 85188-4-RR (same clone as 85188-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85188-4-RR (NSMCE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85188-4-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 85188-4-RR (same clone as 85188-4-PBS)

    IF Staining of U2OS using 85188-4-RR (same clone as 85188-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NSMCE2 antibody (85188-4-RR, Clone: 242862D8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85188-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01897-2

    Sandwich ELISA standard curve of MP01897-2

    Sandwich ELISA standard curve of MP01897-2, Human NSMCE2 Recombinant Matched Antibody Pair - PBS only. 85188-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4545. 85188-5-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01897-2

    Sample test of MP01897-2

    The mean NSMCE2 concentration was determined to be 137.24 ng/mL in HeLa cell extract based on a 1.50 mg/mL extract load, 371.47 ng/mL in Jurkat cell extract based on a 1.90 mg/mL extract load and 481.66 ng/mL in U2OS cell extract based on a 3.50 mg/mL extract load.

  • Affinity and Kinetic Characterization of 85188-4-RR

    Affinity and Kinetic Characterization of 85188-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85188-4-RR against Human NSMCE2 were performed. The affinity constant is below 1 pM.

NSMCE2 Recombinant antibody, PBS Only (Capture)

NSMCE2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85188-4-PBS

产品说明书

CloneNo. 242862D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

别名

EC:2.3.2.-, E3 SUMO-protein transferase NSE2, E3 SUMO-protein ligase NSE2, E3 SUMO protein ligase NSE2, C8orf36

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

85188-4-PBS targets NSMCE2 as part of a matched antibody pair:

MP01897-2: 85188-4-PBS capture and 85188-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 NSMCE2 fusion protein Ag4545 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 non-SMC element 2, MMS21 homolog (S. cerevisiae)
别名 EC:2.3.2.-, E3 SUMO-protein transferase NSE2, E3 SUMO-protein ligase NSE2, E3 SUMO protein ligase NSE2, C8orf36
计算分子量 247 aa, 28 kDa
观测分子量28-32 kDa
GenBank蛋白编号BC032797
基因名称 NSMCE2
Gene ID (NCBI) 286053
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96MF7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NSMCE2 (E3 SUMO-protein ligase NSE2) has a C-terminal Siz/PIAS-RING (SP-RING) domain with E3 SUMO ligase activity (PMID: 15601841). NSMCE2, an essential SUMO ligase of the SMC5/6 complex suppresses cancer and aging in mice (PMID: 26443207).