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  • Featured Product
  • KD/KO Validated

NSUN2 Recombinant antibody

NSUN2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230186G2

Cat No : 82894-2-RR

Print datasheet

Synonyms

FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4

验证数据展示

  • WB analysis using 82894-2-RR

    WB analysis using 82894-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82894-2-RR (NSUN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82894-2-RR

    WB analysis of HeLa using 82894-2-RR

    WB result of NSUN2 antibody (82894-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSUN2 transfected HeLa cells.

  • IF Staining of HepG2 using 82894-2-RR

    IF Staining of HepG2 using 82894-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-2-RR, Clone: 230186G2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, HT-1080 cells
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82894-2-RR targets NSUN2 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen NSUN2 fusion protein Ag14791 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 2
Synonyms FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4
Calculated Molecular Weight 767 aa, 86 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08J23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

实验方案

Product Specific Protocols
WB protocol for NSUN2 antibody 82894-2-RRDownload protocol
IF protocol for NSUN2 antibody 82894-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols