验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, HT-1080 cells, COLO 320 cells, HEK-293 cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82894-7-RR targets NSUN2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | NSUN2 fusion protein Ag14791 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | NOL1/NOP2/Sun domain family, member 2 |
别名 | RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.- |
计算分子量 | 767 aa, 86 kDa |
观测分子量 | 90-100 kDa |
GenBank蛋白编号 | BC001041 |
基因名称 | NSUN2 |
Gene ID (NCBI) | 54888 |
RRID | AB_3670623 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q08J23 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.
实验方案
Product Specific Protocols | |
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WB protocol for NSUN2 antibody 82894-7-RR | Download protocol |
IHC protocol for NSUN2 antibody 82894-7-RR | Download protocol |
IF protocol for NSUN2 antibody 82894-7-RR | Download protocol |
FC protocol for NSUN2 antibody 82894-7-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |