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NSUN2 Recombinant antibody

NSUN2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230186H8

Cat no : 82894-7-RR

Print datasheet

Synonyms

RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-

验证数据展示

  • WB analysis using 82894-7-RR

    WB analysis using 82894-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82894-7-RR (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 82894-7-RR

    IHC staining of human stomach cancer using 82894-7-RR

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82894-7-RR (NSUN2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NSUN2 antibody included
  • IF Staining of HepG2 using 82894-7-RR

    IF Staining of HepG2 using 82894-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-7-RR, Clone: 230186H8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).CL594-Phalloidin (red).

  • FC experiment of HeLa using 82894-7-RR

    FC experiment of HeLa using 82894-7-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NSUN2 Recombinant antibody (82894-7-RR, Clone:230186H8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82894-7-RR

    Affinity and Kinetic Characterization of 82894-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82894-7-RR against Human NSUN2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HT-1080 cells, COLO 320 cells, HEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NSUN2 Ready-To-Use IHC Kit. NSUN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82894-7-RR targets NSUN2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen NSUN2 fusion protein Ag14791 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 2
Synonyms RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-
Calculated Molecular Weight 767 aa, 86 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08J23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

实验方案

Product Specific Protocols
WB protocol for NSUN2 antibody 82894-7-RRDownload protocol
IHC protocol for NSUN2 antibody 82894-7-RRDownload protocol
IF protocol for NSUN2 antibody 82894-7-RRDownload protocol
FC protocol for NSUN2 antibody 82894-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols