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NSUN4 Recombinant antibody

NSUN4 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241115B7

Cat no : 84275-1-RR

Print datasheet

Synonyms

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 241115B7

验证数据展示

  • WB analysis using 84275-1-RR

    WB analysis using 84275-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84275-1-RR (NSUN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HT-29 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84275-1-RR is NSUN4 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen NSUN4 fusion protein Ag31828 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 4
Synonyms RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 241115B7
Calculated Molecular Weight 384 aa, 43 kDa
Observed Molecular Weight~43 kDa
GenBank Accession NumberBC014441
Gene Symbol NSUN4
Gene ID (NCBI) 387338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96CB9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly.

实验方案

Product Specific Protocols
WB protocol for NSUN4 antibody 84275-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols