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验证数据展示

  • IF Staining of HeLa using 84768-2-RR (same clone as 84768-2-PBS)

    IF Staining of HeLa using 84768-2-RR (same clone as 84768-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NSUN5 antibody (84768-2-RR, Clone: 242168D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84768-2-PBS in a different storage buffer formulation.

  • WB analysis using 84768-2-RR (same clone as 84768-2-PBS)

    WB analysis using 84768-2-RR (same clone as 84768-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84768-2-RR (NSUN5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84768-2-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 84768-2-RR (same clone as 84768-2-PBS)

    IF Staining of A431 using 84768-2-RR (same clone as 84768-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NSUN5 antibody (84768-2-RR, Clone: 242168D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84768-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01548-1

    Cytometric bead array standard curve of MP01548-1

    Cytometric bead array standard curve of MP01548-1, NSUN5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84768-1-PBS. Detection antibody: 84768-2-PBS. Standard: Ag7711. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84768-2-RR

    Affinity and Kinetic Characterization of 84768-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84768-2-RR against Human NSUN5 were performed. The affinity constant is 0.69 nM.

View 3D epitope

NSUN5 Recombinant antibody, PBS Only (Detector)

NSUN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84768-2-PBS

产品说明书

CloneNo. 242168D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

NOL1-related protein, NOL1R, NOL1, EC:2.1.1.-, 28S rRNA (cytosine-C(5))-methyltransferase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 
100 μg

¥5800/ 100 μg

浓度: 1 mg/ml

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产品信息

84768-2-PBS targets NSUN5 as part of a matched antibody pair:

MP01548-1: 84768-1-PBS capture and 84768-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 NSUN5 fusion protein Ag7711 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NOL1/NOP2/Sun domain family, member 5
别名 NOL1-related protein, NOL1R, NOL1, EC:2.1.1.-, 28S rRNA (cytosine-C(5))-methyltransferase
计算分子量 47 kDa
观测分子量47~51 kDa
GenBank蛋白编号BC008084
基因名称 NSUN5
Gene ID (NCBI) 55695
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96P11
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NSUN5 is a conserved RNA methyltransferase belonging to the Nop2/SUN domain family, which is highly conserved from archaea to eukaryotes and has close contact with RNA methylation because of their S-adenosyl methionine binding-domain. NSUN5 was a promoter in the progression of CRC mainly through cell cycle regulation (PMID: 32774740). Loss of NSUN5 induces growth phenotypes in mice and different mammalian cells, which is likely connected to the requirement of NSUN5 for ribosomal RNA modification and normal global protein translation, especially in highly proliferative cells (PMID: 31722427). Western blot analysis detected NSUN5 at an apparent molecular mass of 47 kDa.