验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, HEK-293 cells, HepG2 cells, mouse brain |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NSUN6 Ready-To-Use IHC Kit. NSUN6 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17240-1-AP targets NSUN6 in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, RIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | NSUN6 fusion protein Ag11084 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | NOL1/NOP2/Sun domain family, member 6 |
| Synonyms | 4933414E04Rik, NOPD1, NSUN6 |
| Calculated Molecular Weight | 469 aa, 52 kDa |
| Observed Molecular Weight | 52-55 kDa |
| GenBank Accession Number | BC035778 |
| Gene Symbol | NSUN6 |
| Gene ID (NCBI) | 221078 |
| RRID | AB_2878367 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TEA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSUN6 is an RNA methyltransferase that catalyzes the methylation at position cytosine C72 in some tRNAs. NSUN6 has been reported to localize to the cytoplasm and largely colocalize with Golgi apparatus and pericentriolar matrix, indicating that NSUN6 modifies tRNAs in a late step in their biogenesis. NSUN6 may also play a major role in bone metastasis and could be a valuable therapeutic target for bone metastasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NSUN6 antibody 17240-1-AP | Download protocol |
| IHC protocol for NSUN6 antibody 17240-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach | ||
Nucleic Acids Res Sequence- and structure-specific cytosine-5 mRNA methylation by NSUN6.
| ||
Mol Syst Biol CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators.
| ||
Mol Cancer NSUN6-mediated 5-methylcytosine modification of NDRG1 mRNA promotes radioresistance in cervical cancer
| ||
Cell Rep STUB1-mediated ubiquitination and degradation of NSUN2 promotes hepatocyte ferroptosis by decreasing m5C methylation of Gpx4 mRNA |