验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, HEK-293 cells, HepG2 cells, mouse brain |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NSUN6 Ready-To-Use IHC Kit. NSUN6 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17240-1-AP targets NSUN6 in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | NSUN6 fusion protein Ag11084 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NOL1/NOP2/Sun domain family, member 6 |
| 别名 | 4933414E04Rik, NOPD1, NSUN6 |
| 计算分子量 | 469 aa, 52 kDa |
| 观测分子量 | 52-55 kDa |
| GenBank蛋白编号 | BC035778 |
| 基因名称 | NSUN6 |
| Gene ID (NCBI) | 221078 |
| RRID | AB_2878367 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TEA1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSUN6 is an RNA methyltransferase that catalyzes the methylation at position cytosine C72 in some tRNAs. NSUN6 has been reported to localize to the cytoplasm and largely colocalize with Golgi apparatus and pericentriolar matrix, indicating that NSUN6 modifies tRNAs in a late step in their biogenesis. NSUN6 may also play a major role in bone metastasis and could be a valuable therapeutic target for bone metastasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NSUN6 antibody 17240-1-AP | Download protocol |
| IHC protocol for NSUN6 antibody 17240-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach | ||
Nucleic Acids Res Sequence- and structure-specific cytosine-5 mRNA methylation by NSUN6.
| ||
Mol Syst Biol CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators.
| ||
Mol Cancer NSUN6-mediated 5-methylcytosine modification of NDRG1 mRNA promotes radioresistance in cervical cancer
| ||
Cell Rep STUB1-mediated ubiquitination and degradation of NSUN2 promotes hepatocyte ferroptosis by decreasing m5C methylation of Gpx4 mRNA |