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NSUN6 Monoclonal antibody

NSUN6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H1H8

Cat no : 68614-1-Ig

Print datasheet

Synonyms

4933414E04Rik, FLJ23743, NOPD1, NSUN6

验证数据展示

  • WB analysis using 68614-1-Ig

    WB analysis using 68614-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68614-1-Ig (NSUN6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, Mo7e cells, Ramos cells, Raji cells, pig liver tissue
Planning an IHC experiment? We recommend our IHCeasy NSUN6 Ready-To-Use IHC Kit. NSUN6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68614-1-Ig targets NSUN6 in WB, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, pig
Immunogen NSUN6 fusion protein Ag11084 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 6
Synonyms 4933414E04Rik, FLJ23743, NOPD1, NSUN6
Calculated Molecular Weight 469 aa, 52 kDa
Observed Molecular Weight50-52 kDa
GenBank Accession NumberBC035778
Gene Symbol NSUN6
Gene ID (NCBI) 221078
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TEA1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN6 is an RNA methyltransferase that catalyzes the methylation at position cytosine C72 in some tRNAs. NSUN6 has been reported to localize to the cytoplasm and largely colocalize with the Golgi apparatus and pericentriolar matrix, indicating that NSUN6 modifies tRNAs in a late step in their biogenesis. NSUN6 may also play a major role in bone metastasis and could be a valuable therapeutic target for bone metastasis.

实验方案

Product Specific Protocols
WB protocol for NSUN6 antibody 68614-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols