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Host
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Species Reactivity
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Species Reactivity
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验证数据展示

  • WB analysis using 17546-1-AP

    WB analysis using 17546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17546-1-AP (NSUN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 17546-1-AP

    WB analysis using 17546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17546-1-AP (NSUN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 17546-1-AP

    IHC staining of rat testis using 17546-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17546-1-AP (NSUN7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NSUN7 Polyclonal antibody

NSUN7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17546-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

FLJ14001

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse testis tissue, rat liver tissue
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17546-1-AP targets NSUN7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NSUN7 fusion protein Ag11650 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 7
Synonyms FLJ14001
Calculated Molecular Weight 718 aa, 81 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC036568
Gene Symbol NSUN7
Gene ID (NCBI) 79730
RRIDAB_2878415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NE18
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163).

实验方案

Product Specific Protocols
WB protocol for NSUN7 antibody 17546-1-APDownload protocol
IHC protocol for NSUN7 antibody 17546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Comprehensive Analysis of 5-Methylcytosine (m5C) Regulators and the Immune Microenvironment in Pancreatic Adenocarcinoma to Aid Immunotherapy.

Authors - Ronglin Wang
View Article