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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 29787-1-AP

    WB analysis using 29787-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29787-1-AP (NSUN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

NSUN7 Polyclonal antibody

NSUN7 Polyclonal Antibody for WB, ELISA
Cat No. 29787-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

FLJ14001

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29787-1-AP targets NSUN7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NSUN7 fusion protein Ag31860 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NOL1/NOP2/Sun domain family, member 7
Synonyms FLJ14001
Calculated Molecular Weight 718 aa, 81 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC036568
Gene Symbol NSUN7
Gene ID (NCBI) 79730
RRIDAB_2923609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163).

实验方案

Product Specific Protocols
WB protocol for NSUN7 antibody 29787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols