验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84795-1-RR targets NSUN7 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human, rat |
| Immunogen | NSUN7 fusion protein Ag11650 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | NOL1/NOP2/Sun domain family, member 7 |
| Synonyms | FLJ14001, 242358B7 |
| Calculated Molecular Weight | 718 aa, 81 kDa |
| Observed Molecular Weight | 81~90 kDa |
| GenBank Accession Number | BC036568 |
| Gene Symbol | NSUN7 |
| Gene ID (NCBI) | 79730 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NE18 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NSUN7 antibody 84795-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |