验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21284-1-AP targets NT5C1A in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | NT5C1A fusion protein Ag15802 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 5'-nucleotidase, cytosolic IA |
| 别名 | 5' nucleotidase, cytosolic IA, CN I, CN IA, CN1, CN1A, Cytosolic 5 nucleotidase 1A, Cytosolic 5 nucleotidase IA, NT5C1A |
| 计算分子量 | 368 aa, 41 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC103880 |
| 基因名称 | NT5C1A |
| Gene ID (NCBI) | 84618 |
| RRID | AB_3085649 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BXI3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NT5C1A (5′-nucleotidase cytosolic 1A) is a 365 amino acid protein belonging to the 5′-nucleotidase type 3 family. AMP can be deaminated by AMP-deaminase (AMPD) to IMP, which is hydrolyzed to inosine by cytosolic 5'-nucleotidase II (NT5C2). AMP can also be hydrolyzed to adenosine by cytosolic 5'-nucleotidase 1A (NT5C1A). NT5C1A also assists in the regulation of adenosine levels in heart during ischemia and hypoxia.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NT5C1A antibody 21284-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |