验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, human placenta tissue, A549 cells, NCl-H1299 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, NCI-H1299 cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IHC | See 1 publications below |
产品信息
15223-1-AP targets NT5C2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | NT5C2 fusion protein Ag7438 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | 5'-nucleotidase, cytosolic II |
别名 | Cytosolic IMP/GMP-specific 5'-nucleotidase, Cytosolic 5'-nucleotidase II, Cytosolic 5 nucleotidase II, cN-II, cN II |
计算分子量 | 65 kDa |
观测分子量 | 60-65 kDa |
GenBank蛋白编号 | BC001595 |
基因名称 | NT5C2 |
Gene ID (NCBI) | 22978 |
RRID | AB_2153946 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P49902 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for NT5C2 antibody 15223-1-AP | Download protocol |
IF protocol for NT5C2 antibody 15223-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
BMC Med Genomics Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis. | ||
Cell Death Dis Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation |