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NT5C2 Polyclonal antibody

NT5C2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15223-1-AP

Print datasheet

Synonyms

Cytosolic IMP/GMP-specific 5'-nucleotidase, Cytosolic 5'-nucleotidase II, Cytosolic 5 nucleotidase II, cN-II, cN II

验证数据展示

  • WB analysis using 15223-1-AP

    WB analysis using 15223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15223-1-AP (NT5C2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 15223-1-AP

    WB analysis of A549 using 15223-1-AP

    WB result of NT5C2 antibody (15223-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5C2 transfected A549 cells.

  • IF Staining of HepG2 using 15223-1-AP

    IF Staining of HepG2 using 15223-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using NT5C2 antibody (15223-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, human placenta tissue, A549 cells, NCl-H1299 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, NCI-H1299 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15223-1-AP targets NT5C2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NT5C2 fusion protein Ag7438 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 5'-nucleotidase, cytosolic II
Synonyms Cytosolic IMP/GMP-specific 5'-nucleotidase, Cytosolic 5'-nucleotidase II, Cytosolic 5 nucleotidase II, cN-II, cN II
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC001595
Gene Symbol NT5C2
Gene ID (NCBI) 22978
RRIDAB_2153946
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49902
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NT5C2 antibody 15223-1-APDownload protocol
IF protocol for NT5C2 antibody 15223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

BMC Med Genomics

Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis.

Authors - Xing-Cheng Zhao
View Article
mouseWB,IHC

Cell Death Dis

Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation

Authors - Mei-Xin Li
View Article