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NT5C3 Polyclonal antibody

NT5C3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11393-1-AP

Print datasheet

Synonyms

5' nucleotidase, cytosolic III, cN III, Cytosolic 5 nucleotidase 3, Cytosolic 5 nucleotidase III, NT5C3, p36, P5N 1, P5'N 1, P5N1, PN I, PSN1, Pyrimidine 5 nucleotidase 1, UMPH, UMPH1

验证数据展示

  • WB analysis of human skeletal muscle using 11393-1-AP

    WB analysis of human skeletal muscle using 11393-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11393-1-AP (NT5C3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 11393-1-AP

    IP experiment of mouse kidney using 11393-1-AP

    IP result of anti-NT5C3 (IP:11393-1-AP, 4ug; Detection:11393-1-AP 1:500) with mouse kidney tissue lysate 3000 ug.

  • IHC staining of human colon cancer using 11393-1-AP

    IHC staining of human colon cancer using 11393-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11393-1-AP (NT5C3 antibody) at dilution of 1:50 (under 10x lens).

经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, mouse kidney tissue, mouse placenta tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11393-1-AP targets NT5C3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen NT5C3 fusion protein Ag1949 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 5'-nucleotidase, cytosolic III
Synonyms 5' nucleotidase, cytosolic III, cN III, Cytosolic 5 nucleotidase 3, Cytosolic 5 nucleotidase III, NT5C3, p36, P5N 1, P5'N 1, P5N1, PN I, PSN1, Pyrimidine 5 nucleotidase 1, UMPH, UMPH1
Calculated Molecular Weight 297 aa, 34 kDa
Observed Molecular Weight 33-38 kDa
GenBank Accession NumberBC015856
Gene Symbol NT5C3
Gene ID (NCBI) 51251
RRIDAB_2267361
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0P0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NT5C3 antibody 11393-1-APDownload protocol
IHC protocol for NT5C3 antibody 11393-1-APDownload protocol
IP protocol for NT5C3 antibody 11393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Biotechnol

Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution

Authors - Qing Dai
View Article