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NT5M Polyclonal antibody

NT5M Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20765-1-AP

Print datasheet

Synonyms

dNT-2, DNT2, dNT 2, Deoxy-5'-nucleotidase 2, 5',3'-nucleotidase, mitochondrial

验证数据展示

  • WB analysis of mouse brain using 20765-1-AP

    WB analysis of mouse brain using 20765-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20765-1-AP (NT5M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skeletal muscle using 20765-1-AP

    IP experiment of mouse skeletal muscle using 20765-1-AP

    IP result of anti-NT5M (IP:20765-1-AP, 4ug; Detection:20765-1-AP 1:600) with mouse skeletal muscle tissue lysate 2200ug.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20765-1-AP targets NT5M in WB, IP, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity mouse
Immunogen NT5M fusion protein Ag14743 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 5',3'-nucleotidase, mitochondrial
Synonyms dNT-2, DNT2, dNT 2, Deoxy-5'-nucleotidase 2, 5',3'-nucleotidase, mitochondrial
Calculated Molecular Weight 228 aa, 26 kDa
Observed Molecular Weight 26-30 kDa
GenBank Accession NumberBC035838
Gene Symbol NT5M
Gene ID (NCBI) 56953
RRIDAB_10733338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPB1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NT5M antibody 20765-1-APDownload protocol
IP protocol for NT5M antibody 20765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols