验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, human placenta tissue |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18084-1-AP targets Neurotrophin 3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Neurotrophin 3 fusion protein Ag12798 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | neurotrophin 3 |
| 别名 | NTF3, NTF 3, NT-3, NT3, NT 3 |
| 计算分子量 | 257 aa, 29 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC069773 |
| 基因名称 | Neurotrophin 3 |
| Gene ID (NCBI) | 4908 |
| RRID | AB_2298715 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20783 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neurotrophin-3 (NT-3) is a member of the neurotrophin family, which are growth factor proteins that play essential roles in the development and maintenance of the nervous system. NT-3 is synthesized by fibroblasts and has been extensively studied for its role in the development of neurons and neural crest-derived cells, such as melanocytes. It can bind to each of the Trk receptors (Trk-A, Trk-B, and Trk-C), but it primarily functions by binding to Trk-C, modulating intracellular signal transduction through the MAPK and PI3K-Akt pathways, which regulate melanocyte differentiation and survival.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Neurotrophin 3 antibody 18084-1-AP | Download protocol |
| IF protocol for Neurotrophin 3 antibody 18084-1-AP | Download protocol |
| IP protocol for Neurotrophin 3 antibody 18084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy. | ||
Stem Cell Res Ther NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model. | ||
Invest Ophthalmol Vis Sci Alteration of Neurotrophic Factors and Innervation in Extraocular Muscles of Individuals With Concomitant Esotropia | ||
Front Pharmacol Synergic Neuroprotection Between Ligusticum Chuanxiong Hort and Borneol Against Ischemic Stroke by Neurogenesis via Modulating Reactive Astrogliosis and Maintaining the Blood-Brain Barrier. | ||
Brain Res Bull PPARα contributes to the therapeutic effect of hydrogen gas against sepsis-associated encephalopathy with the regulation to the CREB-BDNF signaling pathway and hippocampal neuron plasticity-related gene expression. | ||
Neurochem Res Human Umbilical Cord Mesenchymal Stem Cells Overexpressing Nerve Growth Factor Ameliorate Diabetic Cystopathy in Rats. |
