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NTHL1 Polyclonal antibody

NTHL1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 14918-1-AP

Print datasheet

Synonyms

NTH1, NTHL1, OCTS3

验证数据展示

  • WB analysis using 14918-1-AP

    WB analysis using 14918-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14918-1-AP (NTHL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Y79 using 14918-1-AP

    IP experiment of Y79 using 14918-1-AP

    IP result of anti-NTHL1 (IP:14918-1-AP, 4ug; Detection:14918-1-AP 1:500) with Y79 cells lysate 2000ug.

经过测试的应用

Positive WB detected inHEK-293 cells, Y79 cells, HeLa cells, K-562 cells
Positive IP detected inY79 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14918-1-AP targets NTHL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NTHL1 fusion protein Ag6734 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nth endonuclease III-like 1 (E. coli)
Synonyms NTH1, NTHL1, OCTS3
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000391
Gene Symbol NTHL1
Gene ID (NCBI) 4913
RRIDAB_2154555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78549
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

实验方案

Product Specific Protocols
WB protocol for NTHL1 antibody 14918-1-APDownload protocol
IP protocol for NTHL1 antibody 14918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Breakage of cytoplasmic chromosomes by pathological DNA base excision repair.

Authors - Shangming Tang
View Article