验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse brain tissue, rat heart tissue, rat kidney tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
20235-1-AP targets NTN1-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | netrin 1 |
| Synonyms | NTN1, NTN1L, Netrin-1, netrin 1, Epididymis tissue protein Li 131P |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 65-68 kDa |
| GenBank Accession Number | NM_004822 |
| Gene Symbol | NTN1 |
| Gene ID (NCBI) | 9423 |
| RRID | AB_2918065 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95631 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NTN1 is also named as NTN1L. Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. NTN1 is involved in colorectal tumorigenesis by regulating apoptosis. 20235-1-AP antibody detects 65-68 kDa (3 kDa signal peptide) protein in SDS-PAGE. This antibody is specific to NTN1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NTN1-Specific antibody 20235-1-AP | Download protocol |
| IHC protocol for NTN1-Specific antibody 20235-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Diabetes Res High-Glucose-Induced Injury to Proximal Tubules of the Renal System Is Alleviated by Netrin-1 Suppression of Akt/mTOR | ||
Int J Oncol Netrin‑1 interference potentiates epithelial‑to‑mesenchymal transition through the PI3K/AKT pathway under the hypoxic microenvironment conditions of non‑small cell lung cancer. | ||
J Int Med Res Detection of netrin-1 as a novel biomarker for diagnosis and chemotherapeutic monitoring of lung cancer. | ||
Tissue Cell Exosomes derived from human umbilical cord mesenchymal stem cells loaded with RVG-Lamp2b and Netrin-1 promotes Schwann cell invasion and migration | ||
J Ethnopharmacol Novel mechanisms of Anshen Dingzhi prescription against PTSD: Inhibiting DCC to modulate synaptic function and inflammatory responses |
