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验证数据展示

  • WB analysis using 84849-4-RR

    WB analysis using 84849-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84849-4-RR (NUAK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84849-4-RR

    Affinity and Kinetic Characterization of 84849-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84849-4-RR against Human NUAK2 were performed. The affinity constant is below 1 pM.

NUAK2 Recombinant antibody

NUAK2 Recombinant Antibody for WB, ELISA
Cat No. 84849-4-RR

产品说明书

CloneNo. 242220C1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

OMPHK2, NUAK family SNF1-like kinase 2, NUAK 2, EC:2.7.11.1, ANPH2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, SGC-7901 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84849-4-RR targets NUAK2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 NUAK2 fusion protein Ag35302 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NUAK family, SNF1-like kinase, 2
别名 OMPHK2, NUAK family SNF1-like kinase 2, NUAK 2, EC:2.7.11.1, ANPH2
计算分子量70kDa
观测分子量70 kDa
GenBank蛋白编号NM_030952
基因名称 NUAK2
Gene ID (NCBI) 81788
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H093
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUAK2, also known as OMPHK2 and SNARK, is the fourth member of the AMPK kinase family. The molecular mass of NUAK2 is 69 kDa and it plays a key role in neural tube closure during embryonic development (PMID: 32845958).

实验方案

Product Specific Protocols
WB protocol for NUAK2 antibody 84849-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols