验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Raji cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14343-1-AP targets NUB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | NUB1 fusion protein Ag5668 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | negative regulator of ubiquitin-like proteins 1 |
| 别名 | NYREN18, Renal carcinoma antigen NY-REN-18, NUB1L, NUB 1, Negative regulator of ubiquitin-like proteins 1 |
| 计算分子量 | 71 kDa |
| 观测分子量 | 69 kDa |
| GenBank蛋白编号 | BC046354 |
| 基因名称 | NUB1 |
| Gene ID (NCBI) | 51667 |
| RRID | AB_2155889 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5A7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Proteasome-associated protein NEDD8 ultimate buster 1 (NUB1) is an interferon-inducible protein that down-regulates NEDD8 expression and its conjugation system. Over-expression of NUB1 induces a growth-inhibitory effect in cells, and it mediates anti-proliferative actions and apoptosis in renal cell carcinoma cells through cell-cycle regulation. Expression of NUB1 leads to decreased modification of p53 with NEDD8 and stimulation of p53 ubiquitination. Recent finding revealed that NUB1, by regulating GSK3β levels, modulates tau phosphorylation and aggregation, and is a key player in neurodegeneration associated with tau pathology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUB1 antibody 14343-1-AP | Download protocol |
| IF protocol for NUB1 antibody 14343-1-AP | Download protocol |
| IP protocol for NUB1 antibody 14343-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun The role of NUB1 in α-synuclein degradation in Lewy body disease model mice. | ||
Neuropathology Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. | ||
Brain Pathol Phosphorylated NUB1 distinguishes α-synuclein in Lewy bodies from that in glial cytoplasmic inclusions in multiple system atrophy |