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NUBP1 Polyclonal antibody

NUBP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18011-1-AP

Print datasheet

Synonyms

Nucleotide binding protein 1, Nbp35, NBP1, NBP 1, NBP

验证数据展示

  • WB analysis using 18011-1-AP

    WB analysis using 18011-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18011-1-AP (NUBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 18011-1-AP

    WB analysis of HeLa using 18011-1-AP

    WB result of NUBP1 antibody (18011-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUBP1 transfected HeLa cells.

  • IP experiment of Jurkat using 18011-1-AP

    IP experiment of Jurkat using 18011-1-AP

    IP result of anti-NUBP1 (IP:18011-1-AP, 4ug; Detection:18011-1-AP 1:300) with Jurkat cells lysate 3600ug.

  • IHC staining of human stomach using 18011-1-AP

    IHC staining of human stomach using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, LNCaP cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18011-1-AP targets NUBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen NUBP1 fusion protein Ag12510 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleotide binding protein 1 (MinD homolog, E. coli)
Synonyms Nucleotide binding protein 1, Nbp35, NBP1, NBP 1, NBP
Calculated Molecular Weight 309 aa, 33 kDa
Observed Molecular Weight36-40 kDa
GenBank Accession NumberBC100290
Gene Symbol NUBP1
Gene ID (NCBI) 4682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53384
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NUBP1 antibody 18011-1-APDownload protocol
IHC protocol for NUBP1 antibody 18011-1-APDownload protocol
IP protocol for NUBP1 antibody 18011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer

Authors - Subhadip Mukhopadhyay
View Article