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NUBPL Polyclonal antibody

NUBPL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17393-1-AP

Print datasheet

Synonyms

C14orf127, huInd1, IND1 homolog, Iron sulfur protein NUBPL, NUBPL

验证数据展示

  • WB analysis of mouse colon using 17393-1-AP

    WB analysis of mouse colon using 17393-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17393-1-AP (NUBPL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 17393-1-AP

    IHC staining of human colon cancer using 17393-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17393-1-AP (NUBPL antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 17393-1-AP

    IHC staining of human colon cancer using 17393-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17393-1-AP (NUBPL antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse colon tissue, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17393-1-AP targets NUBPL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NUBPL fusion protein Ag11291 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleotide binding protein-like
Synonyms C14orf127, huInd1, IND1 homolog, Iron sulfur protein NUBPL, NUBPL
Calculated Molecular Weight289aa,31 kDa; 319aa,34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC024919
Gene Symbol NUBPL
Gene ID (NCBI) 80224
RRIDAB_2878402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TB37
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NUBPL antibody 17393-1-APDownload protocol
IHC protocol for NUBPL antibody 17393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Heliyon

Disulfidptosis and ferroptosis related genes define the immune microenvironment and NUBPL serves as a potential biomarker for predicting prognosis and immunotherapy response in bladder cancer

Authors - Xuezhou Zhang
View Article