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NUBPL Polyclonal antibody

NUBPL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17393-1-AP

Print datasheet

Synonyms

C14orf127, huInd1, IND1 homolog, Iron sulfur protein NUBPL, NUBPL

验证数据展示

  • WB analysis of mouse colon using 17393-1-AP

    WB analysis of mouse colon using 17393-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17393-1-AP (NUBPL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 17393-1-AP

    IHC staining of human colon cancer using 17393-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17393-1-AP (NUBPL antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 17393-1-AP

    IHC staining of human colon cancer using 17393-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17393-1-AP (NUBPL antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse colon tissue, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

The immunogen of 17393-1-AP is NUBPL Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NUBPL fusion protein Ag11291 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleotide binding protein-like
Synonyms C14orf127, huInd1, IND1 homolog, Iron sulfur protein NUBPL, NUBPL
Calculated Molecular Weight289aa,31 kDa; 319aa,34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC024919
Gene Symbol NUBPL
Gene ID (NCBI) 80224
RRIDAB_2878402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TB37
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NUBPL antibody 17393-1-APDownload protocol
IHC protocol for NUBPL antibody 17393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Heliyon

Disulfidptosis and ferroptosis related genes define the immune microenvironment and NUBPL serves as a potential biomarker for predicting prognosis and immunotherapy response in bladder cancer

Authors - Xuezhou Zhang
View Article