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验证数据展示

  • IF Staining of HepG2 using 83917-5-RR (same clone as 83917-5-PBS)

    IF Staining of HepG2 using 83917-5-RR (same clone as 83917-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUCB2 antibody (83917-5-RR, Clone: 241037C1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83917-5-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83917-5-RR (same clone as 83917-5-PBS)

    FC experiment of A431 using 83917-5-RR (same clone as 83917-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug NUCB2 Recombinant antibody (83917-5-RR, Clone:241037C1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83917-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83917-5-RR (same clone as 83917-5-PBS)

    IF Staining of HepG2 using 83917-5-RR (same clone as 83917-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUCB2/nesfatin-1 antibody (83917-5-RR, Clone: 241037C1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 83917-5-PBS in a different storage buffer formulation.

NUCB2/nesfatin-1 Recombinant antibody, PBS Only

NUCB2/nesfatin-1 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83917-5-PBS

Print datasheet

CloneNo. 241037C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

NUCB2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4, DNA binding protein NEFA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83917-5-PBS targets NUCB2/nesfatin-1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen NUCB2/nesfatin-1 fusion protein Ag25152 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nucleobindin 2
Synonyms NUCB2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4, DNA binding protein NEFA
Calculated Molecular Weight 50 kDa
GenBank Accession NumberNM_005013
Gene Symbol NUCB2
Gene ID (NCBI) 4925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP80303
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).