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验证数据展示

  • WB analysis using 83917-2-RR

    WB analysis using 83917-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83917-2-RR (NUCB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83917-2-RR

    FC experiment of HepG2 using 83917-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR, Clone:241037D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83917-2-RR

    Affinity and Kinetic Characterization of 83917-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83917-2-RR against Human NUCB2/nesfatin-1 were performed. The affinity constant is 8.99 nM.

NUCB2/nesfatin-1 Recombinant antibody

NUCB2/nesfatin-1 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83917-2-RR

产品说明书

CloneNo. 241037D8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

NUCB2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4, DNA binding protein NEFA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, mouse testis tissue, C6 cells, mouse spleen tissue, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHepG2 cells, A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83917-2-RR targets NUCB2/nesfatin-1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NUCB2/nesfatin-1 fusion protein Ag25152 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleobindin 2
别名 NUCB2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4, DNA binding protein NEFA
计算分子量 50 kDa
观测分子量 45-50 kDa
GenBank蛋白编号NM_005013
基因名称 NUCB2
Gene ID (NCBI) 4925
RRIDAB_3671495
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP80303
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).

实验方案

Product Specific Protocols
WB protocol for NUCB2/nesfatin-1 antibody 83917-2-RRDownload protocol
FC protocol for NUCB2/nesfatin-1 antibody 83917-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols