验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, HEK-293 cells, MCF-7 cells, SW 1990 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human gliomas tissue, rat pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12023-2-AP targets NUCKS1 in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC, ChIP |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse, bovine |
| 免疫原 | NUCKS1 fusion protein Ag2652 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear casein kinase and cyclin-dependent kinase substrate 1 |
| 别名 | JC7, NUCKS, NUCKS1, P1 |
| 计算分子量 | 243 aa, 27 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC000805 |
| 基因名称 | NUCKS1 |
| Gene ID (NCBI) | 64710 |
| RRID | AB_906407 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H1E3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 (NUCKS1) is a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated by CDK1 and casein kinase during mitosis of the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Widelyexpressed, with highest levels in thyroid gland, prostate and uterus and in fetal liver, thymus and lung. Two isoforms of NUCKS1 exist due to alternative splicing events.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUCKS1 antibody 12023-2-AP | Download protocol |
| IHC protocol for NUCKS1 antibody 12023-2-AP | Download protocol |
| IP protocol for NUCKS1 antibody 12023-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization | ||
J Exp Clin Cancer Res NUCKS promotes cell proliferation and suppresses autophagy through the mTOR-Beclin1 pathway in gastric cancer.
| ||
J Cell Physiol NUCKS1 is a novel regulator of milk synthesis in and proliferation of mammary epithelial cells via the mTOR signaling pathway. | ||
Cell Death Dis NUCKS1, a LINC00629-upregulated gene, facilitated osteosarcoma progression and metastasis by elevating asparagine synthesis
| ||
Cancer Lett NUCB2 inhibition antagonizes osteosarcoma progression and promotes anti-tumor immunity through inactivating NUCKS1/CXCL8 axis
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