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NUDCD2 Polyclonal antibody

NUDCD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21205-1-AP

Print datasheet

Synonyms

NudCL2, NudC domain-containing protein 2, NudC domain containing 2

验证数据展示

  • WB analysis using 21205-1-AP

    WB analysis using 21205-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21205-1-AP

    IF Staining of HepG2 using 21205-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HepG2 using 21205-1-AP

    IP experiment of HepG2 using 21205-1-AP

    IP result of anti-NUDCD2 (IP:21205-1-AP, 4ug; Detection:21205-1-AP 1:500) with HepG2 cells lysate 1080 ug.

  • WB analysis of human brain using 21205-1-AP

    WB analysis of human brain using 21205-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 21205-1-AP

    IHC staining of human kidney using 21205-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21205-1-AP (NUDCD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 21205-1-AP

    IF Staining of HeLa using 21205-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HepG2 using 21205-1-AP

    IF Staining of HepG2 using 21205-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, human brain tissue, human kidney tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21205-1-AP targets NUDCD2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen NUDCD2 fusion protein Ag15579 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NudC domain containing 2
Synonyms NudCL2, NudC domain-containing protein 2, NudC domain containing 2
Calculated Molecular Weight 157 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC017934
Gene Symbol NUDCD2
Gene ID (NCBI) 134492
RRIDAB_10732725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVJ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).

实验方案

Product Specific Protocols
WB protocol for NUDCD2 antibody 21205-1-APDownload protocol
IHC protocol for NUDCD2 antibody 21205-1-APDownload protocol
IF protocol for NUDCD2 antibody 21205-1-APDownload protocol
IP protocol for NUDCD2 antibody 21205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Mol Life Sci

RetSat stabilizes mitotic chromosome segregation in pluripotent stem cells

Authors - Wanzhi Cai
View Article