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验证数据展示

  • WB analysis using 17508-1-AP

    WB analysis using 17508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17508-1-AP

    IF Staining of HeLa using 17508-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NUDT3/4/10/11 antibody (17508-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NUDT3/4/10/11 Polyclonal antibody

NUDT3/4/10/11 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17508-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

NUDT10, DIPP3A, Diphosphoinositol polyphosphate phosphohydrolase 3-alpha, Diadenosine hexaphosphate hydrolase, Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17508-1-AP targets NUDT3/4/10/11 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NUDT3/4/10/11 fusion protein Ag11711 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 10
别名 NUDT10, DIPP3A, Diphosphoinositol polyphosphate phosphohydrolase 3-alpha, Diadenosine hexaphosphate hydrolase, Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-alpha
计算分子量 164 aa, 19 kDa
观测分子量 23 kDa
GenBank蛋白编号BC050700
基因名称 NUDT10
Gene ID (NCBI) 170685
RRIDAB_2154145
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NFP7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUDT10 (nucleoside diphosphate linked moiety X-type motif 10), also named as DIPP3A or APS2, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).

实验方案

Product Specific Protocols
WB protocol for NUDT3/4/10/11 antibody 17508-1-APDownload protocol
IF protocol for NUDT3/4/10/11 antibody 17508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols