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验证数据展示

  • WB analysis using 17487-1-AP

    WB analysis using 17487-1-AP

    mouse liver tissues were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17487-1-AP

    IF Staining of HeLa using 17487-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17487-1-AP (NUDT12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NUDT12 Polyclonal antibody

NUDT12 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17487-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Nucleoside diphosphate-linked moiety X motif 12, NADH pyrophosphatase NUDT12, NAD-capped RNA hydrolase NUDT12, EC:3.6.1.22, EC:3.6.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, HeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17487-1-AP targets NUDT12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 NUDT12 fusion protein Ag11557 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 12
别名 Nucleoside diphosphate-linked moiety X motif 12, NADH pyrophosphatase NUDT12, NAD-capped RNA hydrolase NUDT12, EC:3.6.1.22, EC:3.6.1.-
计算分子量 462 aa, 52 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC041099
基因名称 NUDT12
Gene ID (NCBI) 83594
RRIDAB_2236164
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQG2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NUDT12 antibody 17487-1-APDownload protocol
IF protocol for NUDT12 antibody 17487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells.

Authors - Hiroaki Konishi
View Article