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验证数据展示

  • IP experiment of THP-1 using 17318-1-AP

    IP experiment of THP-1 using 17318-1-AP

    IP result of anti-NUDT18 (IP:17318-1-AP, 4ug; Detection:17318-1-AP 1:500) with THP-1 cells lysate 2480 ug.

  • IF Staining of THP-1 using 17318-1-AP

    IF Staining of THP-1 using 17318-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using NUDT18 antibody (17318-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

NUDT18 Polyclonal antibody

NUDT18 Polyclonal Antibody for IF/ICC, IP, ELISA
Cat No. 17318-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IP, ELISA

别名

MTH3, Nucleoside diphosphate-linked moiety X motif 18, EC:3.6.1.58, 8-oxo-dGDP phosphatase NUDT18, 7,8-dihydro-8-oxoguanine phosphatase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inTHP-1 cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17318-1-AP targets NUDT18 in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 NUDT18 fusion protein Ag11292 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nudix (nucleoside diphosphate linked moiety X)-type motif 18
别名 MTH3, Nucleoside diphosphate-linked moiety X motif 18, EC:3.6.1.58, 8-oxo-dGDP phosphatase NUDT18, 7,8-dihydro-8-oxoguanine phosphatase
计算分子量 323 aa, 36 kDa
观测分子量36 kDa
GenBank蛋白编号BC016902
基因名称 NUDT18
Gene ID (NCBI) 79873
RRIDAB_3669267
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZVK8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

8-oxo-dGDP phosphatase NUDT18, also known as MTH3, belongs to the Nudix hydrolase family that eliminate potentially toxic nucleotide metabolites from the cell and regulate the concentrations and availability of various nucleotide substrates, cofactors, and signaling molecules (PMID: 35732208, 22556419). NUDT18 is specifically active against 8-oxo-dGDP and hardly cleaves 8-oxo-dGTP. NUDT18 is markedly elevated expression in noncancerous cell lines in comparison to cancerous counterpart, and NUDT18 deficient cells have been reported to exhibit a noticeably reduced proliferation rate (PMID: 38944687).

实验方案

Product Specific Protocols
IF protocol for NUDT18 antibody 17318-1-APDownload protocol
IP protocol for NUDT18 antibody 17318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols