验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, NIH/3T3 cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy NUDT21 Ready-To-Use IHC Kit. NUDT21 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10322-1-AP targets NUDT21 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | NUDT21 fusion protein Ag0393 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nudix (nucleoside diphosphate linked moiety X)-type motif 21 |
| 别名 | Nudix hydrolase 21, Nucleoside diphosphate-linked moiety X motif 21, CPSF5, CPSF25, Cleavage factor Im complex 25 kDa subunit |
| 计算分子量 | 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC001403 |
| 基因名称 | NUDT21 |
| Gene ID (NCBI) | 11051 |
| RRID | AB_2251496 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43809 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| IHC protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| IF protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| IP protocol for NUDT21 antibody 10322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.
| ||
Mol Cell A Complex of U1 snRNP with Cleavage and Polyadenylation Factors Controls Telescripting, Regulating mRNA Transcription in Human Cells.
| ||
Mol Cell Global Promotion of Alternative Internal Exon Usage by mRNA 3' End Formation Factors. | ||
J Exp Med Downregulation of CFIm25 amplifies dermal fibrosis through alternative polyadenylation. | ||
EMBO J The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination. |
