验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, MCF-7 cels, MDA-MB-231 cells, T-47D cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27004-1-AP targets NUDT5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | NUDT5 fusion protein Ag25485 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nudix (nucleoside diphosphate linked moiety X)-type motif 5 |
| 别名 | ADP sugar pyrophosphatase, hYSAH1, Nudix motif 5, NUDIX5, NUDT5, YSA1, YSA1H |
| 计算分子量 | 24 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC000025 |
| 基因名称 | NUDT5 |
| Gene ID (NCBI) | 11164 |
| RRID | AB_3085919 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UKK9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUDT5 (NUDIX hydrolase type 5), also called NUDIX5, is a member of the nudix hydrolase family which eliminate toxic nucleotide derivatives from the cell. NUDT5 expression can affect chromosome remodeling, involved in cell adhesion, cancer stem cell maintenance and epithelial to mesenchyme transition in breast cancer cells. In addtion, the high expression of NUDT5 is probably involved in the poor prognosis of breast cancer, which could be a prognostic factor and potential target in the diagnosis and treatment of breast cancer (PMID: 33571243). This NUDT5 protein can be detected with a molecular weight of 35 kDa (PMID: 33096144).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUDT5 antibody 27004-1-AP | Download protocol |
| IHC protocol for NUDT5 antibody 27004-1-AP | Download protocol |
| IF protocol for NUDT5 antibody 27004-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |