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NUDT5 Polyclonal antibody

NUDT5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27004-1-AP

Print datasheet

Synonyms

ADP sugar pyrophosphatase, hYSAH1, Nudix motif 5, NUDIX5, NUDT5, YSA1, YSA1H

验证数据展示

  • WB analysis using 27004-1-AP

    WB analysis using 27004-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27004-1-AP (NUDT5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 27004-1-AP

    IHC staining of mouse liver using 27004-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27004-1-AP (NUDT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 27004-1-AP

    IF Staining of HeLa using 27004-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT5 antibody (27004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, L02 cells, MCF-7 cels, MDA-MB-231 cells, T-47D cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27004-1-AP targets NUDT5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen NUDT5 fusion protein Ag25485 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 5
Synonyms ADP sugar pyrophosphatase, hYSAH1, Nudix motif 5, NUDIX5, NUDT5, YSA1, YSA1H
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC000025
Gene Symbol NUDT5
Gene ID (NCBI) 11164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKK9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUDT5 (NUDIX hydrolase type 5), also called NUDIX5, is a member of the nudix hydrolase family which eliminate toxic nucleotide derivatives from the cell. NUDT5 expression can affect chromosome remodeling, involved in cell adhesion, cancer stem cell maintenance and epithelial to mesenchyme transition in breast cancer cells. In addtion, the high expression of NUDT5 is probably involved in the poor prognosis of breast cancer, which could be a prognostic factor and potential target in the diagnosis and treatment of breast cancer (PMID: 33571243). This NUDT5 protein can be detected with a molecular weight of 35 kDa (PMID: 33096144).

实验方案

Product Specific Protocols
WB protocol for NUDT5 antibody 27004-1-APDownload protocol
IHC protocol for NUDT5 antibody 27004-1-APDownload protocol
IF protocol for NUDT5 antibody 27004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nucleic Acids Res

Visualization of oxidized guanine nucleotides accumulation in living cells with split MutT

Authors - Yoshihiro Fujikawa
View Article