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NUDT6 Polyclonal antibody
NUDT6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver tissue, mouse brain tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11181-1-AP targets NUDT6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | NUDT6 fusion protein Ag1638 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nudix (nucleoside diphosphate linked moiety X)-type motif 6 |
Synonyms | gfg, FGF2AS, FGF AS, FGF 2, EC:3.6.1.- |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 72 kDa |
GenBank Accession Number | BC009842 |
Gene Symbol | NUDT6 |
Gene ID (NCBI) | 11162 |
RRID | AB_2236190 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P53370 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for NUDT6 antibody 11181-1-AP | Download protocol |
IHC protocol for NUDT6 antibody 11181-1-AP | Download protocol |
IF protocol for NUDT6 antibody 11181-1-AP | Download protocol |
IP protocol for NUDT6 antibody 11181-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Physiol FGF2 gene's antisense protein, NUDT6, plays a depressogenic role by promoting inflammation and suppressing neurogenesis without altering FGF2 signalling | ||
Mol Endocrinol Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2. | ||
Mol Carcinog Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression. | ||
Genes (Basel) Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA.
| ||
Wound Repair Regen GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis. |