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  • KD/KO Validated

NUDT6 Polyclonal antibody

NUDT6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11181-1-AP

Print datasheet

Synonyms

gfg, FGF2AS, FGF AS, FGF 2, EC:3.6.1.-



经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, mouse brain tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NUDT6 Ready-To-Use IHC Kit. NUDT6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11181-1-AP targets NUDT6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen NUDT6 fusion protein Ag1638 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 6
Synonyms gfg, FGF2AS, FGF AS, FGF 2, EC:3.6.1.-
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC009842
Gene Symbol NUDT6
Gene ID (NCBI) 11162
RRIDAB_2236190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53370
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.

实验方案

Product Specific Protocols
WB protocol for NUDT6 antibody 11181-1-APDownload protocol
IHC protocol for NUDT6 antibody 11181-1-APDownload protocol
IF protocol for NUDT6 antibody 11181-1-APDownload protocol
IP protocol for NUDT6 antibody 11181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

J Physiol

FGF2 gene's antisense protein, NUDT6, plays a depressogenic role by promoting inflammation and suppressing neurogenesis without altering FGF2 signalling

Authors - Burak Uzay
humanWB

Mol Endocrinol

Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2.

Authors - MacFarlane Leigh-Ann LA
humanWB

Mol Carcinog

Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression.

Authors - MacFarlane Leigh-Ann LA
mouseWB

Cancer Sci

PMID: 29532562

Authors - Ji-Chang Wang
ratWB

Genes (Basel)

Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA.

Authors - Mark Baguma-Nibasheka
  • KD Validated
humanWB

Wound Repair Regen

GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.

Authors - Xinying Wang