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NUDT8 Polyclonal antibody

NUDT8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16098-1-AP

Print datasheet

Synonyms

Nudix motif 8, NUDT8

验证数据展示

  • WB analysis of Jurkat using 16098-1-AP

    WB analysis of Jurkat using 16098-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 16098-1-AP

    IHC staining of human heart using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human heart using 16098-1-AP

    IHC staining of human heart using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16098-1-AP (NUDT8 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of Jurkat using 16098-1-AP

    WB analysis of Jurkat using 16098-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16098-1-AP (NUDT8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16098-1-AP

    IHC staining of human colon cancer using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16098-1-AP

    IHC staining of human colon cancer using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 16098-1-AP

    IHC staining of mouse heart using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 16098-1-AP

    IHC staining of mouse heart using 16098-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16098-1-AP (NUDT8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman colon cancer tissue, human heart tissue, human kidney tissue, human skin tissue, mouse heart tissue, human liver tissue, human spleen tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16098-1-AP targets NUDT8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NUDT8 fusion protein Ag8945 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 8
Synonyms Nudix motif 8, NUDT8
Calculated Molecular Weight 236 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC018644
Gene Symbol NUDT8
Gene ID (NCBI) 254552
RRIDAB_2154132
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WV74
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NUDT8 antibody 16098-1-APDownload protocol
IHC protocol for NUDT8 antibody 16098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols