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验证数据展示

  • WB analysis of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    WB analysis of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 12515-1-AP (same clone as 12515-1-PBS)

    IHC staining of human ovary tumor using 12515-1-AP (same clone as 12515-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12515-1-AP (NUFIP1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUFIP1 antibody included
  • IHC staining of human ovary tumor using 12515-1-AP (same clone as 12515-1-PBS)

    IHC staining of human ovary tumor using 12515-1-AP (same clone as 12515-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12515-1-AP (NUFIP1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NUFIP1 antibody included
  • IP experiment of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    IP experiment of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    IP result of anti-NUFIP1 (IP:12515-1-AP, 4ug; Detection:12515-1-AP 1:800) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    IF Staining of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUFIP1 antibody (12515-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NUFIP1 Polyclonal antibody, PBS Only

NUFIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12515-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NUFIP1 Ready-To-Use IHC Kit. NUFIP1 primary antibody included.

产品信息

12515-1-PBS targets NUFIP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3197

Product name: Recombinant human NUFIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-276 aa of BC017745

Sequence: MHAPGMKKIKLDTPEEIARWREERRKNYPTLANIERKKKLKLEKEKRGAVLTTTQYGKMKGMSRHSQMAKIRSPGKNHKWKNDNSRQRAVTGSGSHLCDLKLEGPPEANADPLGVLINSDSESDKEEKPQHSVIPKEVTPALCSLMSSYGSLSGSESEPEETPIKTEADVLAENQVLDSSAPKSPSQDVKATVRNFSEAKSENRKKSFEKTNPKRKKDYHNYQTLFEPRTHHPYLLEMLLAPDIRHERNVILQCVRYIIKKDFFGLDTNSAKSKDV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear fragile X mental retardation protein interacting protein 1
别名
计算分子量 56 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC017745
基因名称 NUFIP1
Gene ID (NCBI) 26747
RRIDAB_2298759
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHK0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of FMRP (Fragile X Mental Retardation Protein). FMRP is an RNA binding protein reported to be involved in translational control, notably at postsynaptic sites of protein synthesis as a part of a multiprotein/mRNA complex[PMID:12941608]. NUFIP1 is one of the several FMRP-interacting proteins. NUFIP can act as a pol II-specific basal transcriptional activator in vitro and when ectopically overexpressed in vivo. NUFIP can directly activate promoters by enhancing the ATP-dependent release of hyperphosphorylated form of pol II from open transcription complexes[PMID:15107825].

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