验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells, L02 cells |
Planning an IHC experiment? We recommend our IHCeasy NUFIP2 Ready-To-Use IHC Kit. NUFIP2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17752-1-AP targets NUFIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | NUFIP2 fusion protein Ag12094 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear fragile X mental retardation protein interacting protein 2 |
| 别名 | FIP 82, Cell proliferation-inducing gene 1 protein, 82-FIP, 82 kDa FMRP-interacting protein, 82 FIP |
| 计算分子量 | 695 aa, 76 kDa |
| 观测分子量 | 76-82 kDa |
| GenBank蛋白编号 | BC108307 |
| 基因名称 | NUFIP2 |
| Gene ID (NCBI) | 57532 |
| RRID | AB_2878433 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z417 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUFIP2 antibody 17752-1-AP | Download protocol |
| IHC protocol for NUFIP2 antibody 17752-1-AP | Download protocol |
| IF protocol for NUFIP2 antibody 17752-1-AP | Download protocol |
| IP protocol for NUFIP2 antibody 17752-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. | ||
Proc Natl Acad Sci U S A Succinyl-CoA ligase ADP-forming subunit beta promotes stress granule assembly to regulate redox and drive cancer metastasis | ||
J Cell Biol Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control. | ||
Neuron Increased G3BP2-Tau interaction in tauopathies is a natural defense against Tau aggregation | ||
Cell Rep The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network |
