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NUFIP2 Monoclonal antibody

NUFIP2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A4E3

Cat no : 67195-1-Ig

Print datasheet

Synonyms

182 FIP, 82 FIP, FIP 82, FLJ10976, FMRP interacting protein 2, KIAA1321, NUFIP2, PIG1

验证数据展示

  • WB analysis using 67195-1-Ig

    WB analysis using 67195-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 67195-1-Ig

    IHC staining of mouse cerebellum using 67195-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUFIP2 antibody included
  • IHC staining of mouse cerebellum using 67195-1-Ig

    IHC staining of mouse cerebellum using 67195-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUFIP2 antibody included

经过测试的应用

Positive WB detected inHeLa cells, 4T1 cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NUFIP2 Ready-To-Use IHC Kit. NUFIP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67195-1-Ig targets NUFIP2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen NUFIP2 fusion protein Ag29040 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nuclear fragile X mental retardation protein interacting protein 2
Synonyms 182 FIP, 82 FIP, FIP 82, FLJ10976, FMRP interacting protein 2, KIAA1321, NUFIP2, PIG1
Calculated Molecular Weight 695 aa, 76 kDa
Observed Molecular Weight 76 kDa, 82 kDa
GenBank Accession NumberBC108307
Gene Symbol NUFIP2
Gene ID (NCBI) 57532
RRIDAB_2882489
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z417
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NUFIP2 antibody 67195-1-IgDownload protocol
IHC protocol for NUFIP2 antibody 67195-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols