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NuMA Polyclonal antibody

NuMA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16607-1-AP

Print datasheet

Synonyms

NMP22, NuMA, NuMA protein, NUMA1, SP H antigen

验证数据展示

  • WB analysis of HeLa using 16607-1-AP

    WB analysis of HeLa using 16607-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16607-1-AP (NuMA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HCT 116 using 16607-1-AP

    IF Staining of HCT 116 using 16607-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using NuMA antibody (16607-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human breast cancer using 16607-1-AP

    IHC staining of human breast cancer using 16607-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16607-1-AP (NuMA antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

16607-1-AP targets NuMA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitypig
Immunogen NuMA fusion protein Ag9815 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear mitotic apparatus protein 1
Synonyms NMP22, NuMA, NuMA protein, NUMA1, SP H antigen
Calculated Molecular Weight 238 kDa
Observed Molecular Weight 238 kDa
GenBank Accession NumberBC013023
Gene Symbol NuMA
Gene ID (NCBI) 4926
RRIDAB_2154616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14980
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUMA1, also named as Nuclear matrix protein-22, is a 2115 amino acid protein, which forms multiarm oligomers by association of C-terminal tail domains. NUMA1 resides in the nuclear matrix during interphase. NUMA1 is a highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role and occupies the majority if the nuclear volume. NUMA1 is required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes.

实验方案

Product Specific Protocols
WB protocol for NuMA antibody 16607-1-APDownload protocol
IHC protocol for NuMA antibody 16607-1-APDownload protocol
IF protocol for NuMA antibody 16607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigIF

J Cell Sci

RNA localization to the mitotic spindle is essential for early development and is regulated by kinesin-1 and dynein

Authors - Carolyn M Remsburg
View Article