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验证数据展示

  • WB analysis using 29594-1-AP

    WB analysis using 29594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29594-1-AP (NUMB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 29594-1-AP

    IF Staining of A431 using 29594-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using NUMB antibody (29594-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

NUMB Polyclonal antibody

NUMB Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29594-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Synonyms

h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29594-1-AP targets NUMB in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen NUMB fusion protein Ag31553 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name numb homolog (Drosophila)
Synonyms h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171
Calculated Molecular Weight71KD
Observed Molecular Weight66-72 kDa
GenBank Accession NumberNM_001005743
Gene Symbol NUMB
Gene ID (NCBI) 8650
RRIDAB_2923598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49757
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUMB plays a role in the process of neurogenesis. It is required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. NUMB may also mediate local repair of brain ventricular wall damage. NUMB may play a role infurther understanding the NUMB function in development and cancer.

实验方案

Product Specific Protocols
WB protocol for NUMB antibody 29594-1-APDownload protocol
IF protocol for NUMB antibody 29594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols