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验证数据展示

  • WB analysis using 29594-1-AP

    WB analysis using 29594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29594-1-AP (NUMB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 29594-1-AP

    IF Staining of A431 using 29594-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using NUMB antibody (29594-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

NUMB Polyclonal antibody

NUMB Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29594-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, ELISA

别名

h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29594-1-AP targets NUMB in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 NUMB fusion protein Ag31553 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 numb homolog (Drosophila)
别名 h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171
计算分子量71KD
观测分子量66-72 kDa
GenBank蛋白编号NM_001005743
基因名称 NUMB
Gene ID (NCBI) 8650
RRIDAB_2923598
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49757
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUMB plays a role in the process of neurogenesis. It is required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. NUMB may also mediate local repair of brain ventricular wall damage. NUMB may play a role infurther understanding the NUMB function in development and cancer.

实验方案

Product Specific Protocols
WB protocol for NUMB antibody 29594-1-APDownload protocol
IF protocol for NUMB antibody 29594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols