NUP205 Polyclonal antibody
NUP205 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, Jurkat cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 24439-1-AP is NUP205 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | NUP205 fusion protein Ag19832 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nucleoporin 205kDa |
| Synonyms | 205 kDa nucleoporin, C7orf14, KIAA0225, nucleoporin 205kDa, Nucleoporin Nup205, NUP205 |
| Calculated Molecular Weight | 2012 aa, 228 kDa |
| Observed Molecular Weight | 205 kDa |
| GenBank Accession Number | BC136624 |
| Gene Symbol | NUP205 |
| Gene ID (NCBI) | 23165 |
| RRID | AB_2879550 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92621 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUP205, also named as C7orf14 and KIAA0225, is a 205 kDa nucleoporin. It plays a role in the nuclear pore complex (NPC) assembly and/or maintenance.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUP205 antibody 24439-1-AP | Download protocol |
| IHC protocol for NUP205 antibody 24439-1-AP | Download protocol |
| IP protocol for NUP205 antibody 24439-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Methods Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM). | ||
J Clin Invest Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling. | ||
Oncogene DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity. | ||
Hum Mutat Lamin A mutation impairs interaction with nucleoporin NUP155 and disrupts nucleocytoplasmic transport in atrial fibrillation. | ||
Cell Rep Methods TissUExM enables quantitative ultrastructural analysis in whole vertebrate embryos by expansion microscopy | ||
Front Oncol Exploring the relationship between abnormally high expression of NUP205 and the clinicopathological characteristics, immune microenvironment, and prognostic value of lower-grade glioma |
