NUP214 Polyclonal antibody
NUP214 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NUP214 Ready-To-Use IHC Kit. NUP214 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
24113-1-AP targets NUP214 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Immunogen | NUP214 fusion protein Ag21377 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nucleoporin 214kDa |
| Synonyms | 214 kDa nucleoporin, CAIN, CAN, D9S46E, KIAA0023, N214, nucleoporin 214kDa, Nucleoporin Nup214, NUP214, Protein CAN |
| Calculated Molecular Weight | 2080 aa, 213 kDa |
| Observed Molecular Weight | 250 kDa |
| GenBank Accession Number | BC105998 |
| Gene Symbol | NUP214 |
| Gene ID (NCBI) | 8021 |
| RRID | AB_3085723 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P35658 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUP214 is also known as CAN and is an FG nucleoporin anchored to the cytoplasmic ring of the nuclear pore complex (NPC) and forms a subcomplex with nucleoporin NUP88. NUP214 is a component of the NPC with a key role in protein and mRNA nuclear export. NUP214 is a target for chromosomal translocations involved in leukemogenesis (PMID: 17264208, 30669574). NUP214 is expressed in thymus, spleen, bone marrow, kidney, brain, and testis, but hardly in all other tissues or in whole embryos during development. The observed molecular weight of NUP214 is 250 kDa, which is consistent with the description in the literature (PMID: 12191473).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUP214 antibody 24113-1-AP | Download protocol |
| IHC protocol for NUP214 antibody 24113-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
