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Host
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 24113-1-AP

    WB analysis using 24113-1-AP

    K-562 cells were subjected to Tris-Acetate gel system followed by western blot with 24113-1-AP (NUP214 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 24113-1-AP

    IHC staining of mouse kidney using 24113-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24113-1-AP (NUP214 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUP214 antibody included

NUP214 Polyclonal antibody

NUP214 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24113-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

214 kDa nucleoporin, CAIN, CAN, D9S46E, KIAA0023, N214, nucleoporin 214kDa, Nucleoporin Nup214, NUP214, Protein CAN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NUP214 Ready-To-Use IHC Kit. NUP214 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24113-1-AP targets NUP214 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen NUP214 fusion protein Ag21377 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleoporin 214kDa
Synonyms 214 kDa nucleoporin, CAIN, CAN, D9S46E, KIAA0023, N214, nucleoporin 214kDa, Nucleoporin Nup214, NUP214, Protein CAN
Calculated Molecular Weight 2080 aa, 213 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC105998
Gene Symbol NUP214
Gene ID (NCBI) 8021
RRIDAB_3085723
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP35658
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUP214 is also known as CAN and is an FG nucleoporin anchored to the cytoplasmic ring of the nuclear pore complex (NPC) and forms a subcomplex with nucleoporin NUP88. NUP214 is a component of the NPC with a key role in protein and mRNA nuclear export. NUP214 is a target for chromosomal translocations involved in leukemogenesis (PMID: 17264208, 30669574). NUP214 is expressed in thymus, spleen, bone marrow, kidney, brain, and testis, but hardly in all other tissues or in whole embryos during development. The observed molecular weight of NUP214 is 250 kDa, which is consistent with the description in the literature (PMID: 12191473).

实验方案

Product Specific Protocols
WB protocol for NUP214 antibody 24113-1-APDownload protocol
IHC protocol for NUP214 antibody 24113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,ratWB,IHC

J Ethnopharmacol

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Authors - Liwen Lin
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