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验证数据展示

  • WB analysis using 12024-1-AP (same clone as 12024-1-PBS)

    WB analysis using 12024-1-AP (same clone as 12024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12024-1-AP (NUSAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12024-1-AP (same clone as 12024-1-PBS)

    IP experiment of HeLa using 12024-1-AP (same clone as 12024-1-PBS)

    IP result of anti-NUSAP1 (IP:12024-1-AP, 4ug; Detection:12024-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 12024-1-AP (same clone as 12024-1-PBS)

    IHC staining of human prostate cancer using 12024-1-AP (same clone as 12024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 12024-1-AP (same clone as 12024-1-PBS)

    IHC staining of human prostate cancer using 12024-1-AP (same clone as 12024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12024-1-AP (NUSAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12024-1-AP (same clone as 12024-1-PBS)

    IF Staining of HeLa using 12024-1-AP (same clone as 12024-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUSAP1 antibody (12024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12024-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NUSAP1 Polyclonal antibody, PBS Only

NUSAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12024-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12024-1-PBS targets NUSAP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 NUSAP1 fusion protein Ag2654 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleolar and spindle associated protein 1
别名
计算分子量 440 aa, 49 kDa
观测分子量 47-52 kDa
GenBank蛋白编号BC001308
基因名称 NUSAP1
Gene ID (NCBI) 51203
RRIDAB_2157789
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXS6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.