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验证数据展示

  • WB analysis of K-562 using 15538-1-AP

    WB analysis of K-562 using 15538-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15538-1-AP (NTF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 15538-1-AP

    IF Staining of MCF-7 using 15538-1-AP

    Immunofluorescent analysis of MCF-7 cells using 15538-1-AP (NTF2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary tumor using 15538-1-AP

    IHC staining of human ovary tumor using 15538-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15538-1-AP (NTF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NTF2 Polyclonal antibody

NTF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15538-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

NUTF2, Placental protein 15, NUTF 2, Nuclear transport factor 2, NTF-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15538-1-AP targets NTF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NTF2 fusion protein Ag7879 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear transport factor 2
别名 NUTF2, Placental protein 15, NUTF 2, Nuclear transport factor 2, NTF-2
计算分子量 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC002348
基因名称 NTF2
Gene ID (NCBI) 10204
RRIDAB_2157805
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61970
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUTF2 (nuclear transport factor 2), also named as NTF2, is a cytosolic protein responsible for nuclear import of Ran, a small Ras-like GTPase involved in a number of critical cellular processes, including cell cycle regulation, chromatin organization during mitosis, reformation of the nuclear envelope following mitosis, and controlling the directionality of nucleocytoplasmic transport. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells, and may involve a tyrosine and/or threonine kinase-dependent signal transduction mechanism(s) (PMID: 22880006). The MW of this protein is 14 kDa, and this antibody specially recognises the 14 kDa protein.

实验方案

Product Specific Protocols
WB protocol for NTF2 antibody 15538-1-APDownload protocol
IHC protocol for NTF2 antibody 15538-1-APDownload protocol
IF protocol for NTF2 antibody 15538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols