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NXF2 Polyclonal antibody

NXF2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12185-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 39, CT39, nuclear RNA export factor 2, NXF2, NXF2B, TAP like protein 2, TAPL 2, TAPL2

验证数据展示

  • WB analysis of mouse testis using 12185-1-AP

    WB analysis of mouse testis using 12185-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12185-1-AP (NXF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 12185-1-AP

    IHC staining of human lymphoma using 12185-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 12185-1-AP (NXF2 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of mouse testis using 12185-1-AP

    IP experiment of mouse testis using 12185-1-AP

    IP result of anti-NXF2 (IP:12185-1-AP, 4ug; Detection:12185-1-AP 1:400) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

12185-1-AP targets NXF2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen NXF2 fusion protein Ag2841 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear RNA export factor 2
Synonyms Cancer/testis antigen 39, CT39, nuclear RNA export factor 2, NXF2, NXF2B, TAP like protein 2, TAPL 2, TAPL2
Calculated Molecular Weight 626 aa, 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC015020
Gene Symbol NXF2
Gene ID (NCBI) 56001
RRIDAB_2236531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NXF2, also named as Nuclear RNA export factor 2 or TAPL2, is a 626 amino acid protein, which contains one TAP-C domain, one RRM domain, one NTF2 domain, and four LRR repeats. NXF2 belongs to the NXF family and is expressed almost exclusively in testis.NXF2 localizes in the nucleus and is involved in the export of mRNA from the nucleus to the cytoplasm.

实验方案

Product Specific Protocols
WB protocol for NXF2 antibody 12185-1-APDownload protocol
IHC protocol for NXF2 antibody 12185-1-APDownload protocol
IP protocol for NXF2 antibody 12185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Biochem J

Nuclear export factor 3 is involved in regulating the expression of TGF-β3 in an mRNA export activity-independent manner in mouse Sertoli cells.

Authors - Yin Yimeng Y
View Article