验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, HeLa cells, human heart tissue, HCT 116 cells, HEK-293 cells, HepG2 cells, SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16128-1-AP targets NXN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | NXN fusion protein Ag9113 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nucleoredoxin |
| 别名 | TRG 4, Nucleoredoxin, NRX, EC:1.8.1.8 |
| 计算分子量 | 435 aa, 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC009327 |
| 基因名称 | NXN |
| Gene ID (NCBI) | 64359 |
| RRID | AB_2158114 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6DKJ4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NXN antibody 16128-1-AP | Download protocol |
| IHC protocol for NXN antibody 16128-1-AP | Download protocol |
| IF protocol for NXN antibody 16128-1-AP | Download protocol |
| IP protocol for NXN antibody 16128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Signal Mitochondrial reactive oxygen species promote epidermal differentiation and hair follicle development. | ||
Free Radic Biol Med Oxidative stress as a candidate mechanism for accelerated neuroectodermal differentiation due to trisomy 21. | ||
FASEB J A crucial role for Nox 1 in redox-dependent regulation of Wnt-β-catenin signaling. | ||
Antioxidants (Basel) Enzymatic Depletion of Mitochondrial Inorganic Polyphosphate (polyP) Increases the Generation of Reactive Oxygen Species (ROS) and the Activity of the Pentose Phosphate Pathway (PPP) in Mammalian Cells. | ||
Antioxidants (Basel) Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation.
| ||
Cell Death Dis NXN suppresses metastasis of hepatocellular carcinoma by promoting degradation of Snail through binding to DUB3. |
