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ChromoTek Nano-CaptureLigand® mouse IgG1, Fc-specific VHH, biotinylated

The Nano-CaptureLigand® mouse IgG1 captures and immobilizes mouse IgG1 antibodies and Fc-fragments to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to the mouse IgG1 subclass with high affinity and in a site-directed manner.

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Host

Alpaca

Reactivity

Mouse IgG1

Cat No : smsG1B-1

Synonyms

Nano-CaptureLigand® mouse IgG1, Fc domain, mouse IgG1, mouse immunoglobulin Gamma 1

Validation Data Gallery

  • BLI binding kinetics of a mouse IgG1 anti-DYKDDDDK-tag antibody to a DYKDDDDK-tagged protein. A monoclonal mouse IgG1 anti-DYKDDDDK-tag antibody was immobilized using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of a DYKDDDDK-tagged protein.

    BLI binding kinetics of a mouse IgG1 anti-DYKDDDDK-tag antibody to a DYKDDDDK-tagged protein. A monoclonal mouse IgG1 anti-DYKDDDDK-tag antibody was immobilized using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of a DYKDDDDK-tagged protein.

  • BLI binding kinetics of a monoclonal rabbit IgG anti-DYKDDDDK-tag antibody to a DYKDDDDK-tagged protein. A monoclonal rabbit IgG anti-DYKDDDDK-tag antibody was immobilized using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of a DYKDDDDK-tagged protein.

    BLI binding kinetics of a monoclonal rabbit IgG anti-DYKDDDDK-tag antibody to a DYKDDDDK-tagged protein. A monoclonal rabbit IgG anti-DYKDDDDK-tag antibody was immobilized using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of a DYKDDDDK-tagged protein.

  • BLI binding kinetics of a purified and a hybridoma supernatant mouse IgG1 anti-Halo antibody to Halo-tag. A purified, monoclonal mouse IgG1 anti-Halo antibody (blue, ChromoTek Halo antibody [28A8]) and a hybridoma supernatant, monoclonal mouse IgG1 anti-Halo antibody (green) were immobilized using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of Halo-tag protein.

    BLI binding kinetics of a purified and a hybridoma supernatant mouse IgG1 anti-Halo antibody to Halo-tag. A purified, monoclonal mouse IgG1 anti-Halo antibody (blue, ChromoTek Halo antibody [28A8]) and a hybridoma supernatant, monoclonal mouse IgG1 anti-Halo antibody (green) were immobilized using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of Halo-tag protein.

  • ELISA capture of mouse IgG1 antibody using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated. 50 nM Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Mouse IgG1 antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

    ELISA capture of mouse IgG1 antibody using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated. 50 nM Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Mouse IgG1 antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

  • Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and secondary antibody for read-out (bottom right).

    Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and secondary antibody for read-out (bottom right).

  • Species reactivity of Secondary VHH.

    Species reactivity of Secondary VHH.

Product Information

The Nano-CaptureLigand® mouse IgG1 captures and immobilizes mouse IgG1 antibodies and Fc-fragments to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to the mouse IgG1 subclass with high affinity and in a site-directed manner.

DescriptionThe next level of antibody immobilization to BLI and SPR biosensors

• Gentle, site-directed and Fc-specific immobilization of mouse IgG1 antibodies and Fc-fragments to streptavidin coated biosensors without biotinylation of the antibody

• Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems)

• High affinity for stable baseline without significant dissociation

• >10x regeneration for multiple reuse

• High specificity enables mouse IgG1 antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples

ApplicationsBLI, SPR, ELISA
HostAlpaca
ReactivityMouse IgG1 Fc-fragment
No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG2a, IgG2b, IgG2c, IgG3
ConjugateBiotin
Physical StateLiquid
Purification MethodRecombinant expression, affinity purification IMAC
TypeNanobody
ClassRecombinant
Affinity (KD) KD=0.13 nM
Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).
Storage Buffer25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F
RRIDAB_2848186
NoteThis product is for research use only, not for diagnostic or therapeutic use