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验证数据展示

  • WB analysis using 31026-1-AP

    WB analysis using 31026-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31026-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 31026-1-AP

    IHC staining of human placenta using 31026-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31026-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Nectin-4/PVRL4 antibody included
  • IF Staining of MCF-7 using 31026-1-AP

    IF Staining of MCF-7 using 31026-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Nectin-4/PVRL4 antibody (31026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Nectin-4/PVRL4 Polyclonal antibody

Nectin-4/PVRL4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31026-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Nectin-4, PVRL4, poliovirus receptor related 4, Nectin cell adhesion molecule 4, nectin 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, MCF-7 cells, T-47D cells, MDA-MB-231 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Nectin-4/PVRL4 Ready-To-Use IHC Kit. Nectin-4/PVRL4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31026-1-AP targets Nectin-4/PVRL4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poliovirus receptor-related 4
别名 Nectin-4, PVRL4, poliovirus receptor related 4, Nectin cell adhesion molecule 4, nectin 4
计算分子量 510 aa, 55 kDa
观测分子量55-80 kDa
GenBank蛋白编号BC010423
基因名称 Nectin-4
Gene ID (NCBI) 81607
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96NY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a glycosylated form of 60-80 kDa and a deglycosylated form of 55-70 kDa. The 43 kDa band immunoprecipitated in the supernatant corresponds to the soluble Nectin-4 form. (PMID: 15784625).

实验方案

Product Specific Protocols
WB protocol for Nectin-4/PVRL4 antibody 31026-1-APDownload protocol
IHC protocol for Nectin-4/PVRL4 antibody 31026-1-APDownload protocol
IF protocol for Nectin-4/PVRL4 antibody 31026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols