Neprilysin/CD10 Recombinant antibody
Neprilysin/CD10 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
241322A8
验证数据展示
经过测试的应用
Positive WB detected in | mouse kidney tissue, Raji cells, rat kidney tissue |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse kidney tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84181-1-RR targets Neprilysin/CD10 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Recombinant Protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | membrane metallo-endopeptidase |
Synonyms | MME, Neprilysin, Enkephalinase, EC:3.4.24.11, CD10 |
Calculated Molecular Weight | 750 aa, 86 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC101658 |
Gene Symbol | CD10 |
Gene ID (NCBI) | 4311 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08473 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Neprilysin/CD10 antibody 84181-1-RR | Download protocol |
IHC protocol for Neprilysin/CD10 antibody 84181-1-RR | Download protocol |
IF protocol for Neprilysin/CD10 antibody 84181-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |