验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, 3T3-L1 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23060-1-AP targets NeuN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, hamster, goat |
| 免疫原 | NeuN fusion protein Ag19347 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hexaribonucleotide binding protein 3 |
| 别名 | |
| 观测分子量 | 38-50 kDa |
| GenBank蛋白编号 | BC093713 |
| 基因名称 | NeuN |
| Gene ID (NCBI) | 146713 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A6NFN3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NeuN, also named as FOX3 and RBFOX3, is neuronal-specific nuclear protein with MW 38-50kd(~48kd). It is one of a family of 3 mammalian Fox homologues. FOX3(Or NeuN) functions in RNA-binding protein that regulates alternative splicing events.
发表文章
| Species | Application | Title |
|---|---|---|
Cell Cycle Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells. | ||
Exp Cell Res CEBPD aggravates apoptosis and oxidative stress of neuron after ischemic stroke by Nrf2/HO-1 pathway | ||
Neurochem Int Tert-butylhydroquinone post-treatment attenuates neonatal hypoxic-ischemic brain damage in rats. | ||
J Stroke Cerebrovasc Dis An Increase of Sigma-1 Receptor in the Penumbra Neuron after Acute Ischemic Stroke. | ||
Pediatr Surg Int Pathological changes of interstitial cells of Cajal and ganglion cells in the segment of resected bowel in Hirschsprung's disease. |
