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NeuN Polyclonal antibody

NeuN Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (2)

Applications

WB, IHC, IF-P, FC (Intra), ELISA and More (2)

Conjugate

Unconjugated

Cat no : 26975-1-AP

Print datasheet

Synonyms

RBFOX3, Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3

验证数据展示

  • IF Staining of rat cerebellum using 26975-1-AP

    IF Staining of rat cerebellum using 26975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red).

  • WB analysis using 26975-1-AP

    WB analysis using 26975-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 26975-1-AP

    IF Staining of mouse cerebellum using 26975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 26975-1-AP

    IF Staining of mouse cerebellum using 26975-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:5000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of rat cerebellum using 26975-1-AP

    IHC staining of rat cerebellum using 26975-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with NeuN antibody included
  • FC experiment of U-87 MG using 26975-1-AP

    FC experiment of U-87 MG using 26975-1-AP

    1X10^6 U-87 MG cells were intracellularly stained with 0.2 ug Anti-Human NeuN (26975-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive FC (Intra) detected inU-87 MG cells
Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26975-1-AP targets NeuN in WB, IHC, IF-P, FC (Intra), Dot blot, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF, Dot blot
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig, monkey, zebrafish
Immunogen NeuN fusion protein Ag25689 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hexaribonucleotide binding protein 3
Synonyms RBFOX3, Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3
Observed Molecular Weight 46-52 kDa
GenBank Accession NumberNM_001082575
Gene Symbol NeuN
Gene ID (NCBI) 146713
RRIDAB_2880708
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Function

NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).

Tissue specificity

NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).

Involvement in disease

· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).

Isoforms

There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).

Post-translational modifications

Currently not known.

Cellular localization

NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).


实验方案

Product Specific Protocols
WB protocol for NeuN antibody 26975-1-APDownload protocol
IHC protocol for NeuN antibody 26975-1-APDownload protocol
IF protocol for NeuN antibody 26975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Immunity

Fate mapping of Spp1 expression reveals age-dependent plasticity of disease-associated microglia-like cells after brain injury

Authors - Yangning Lan
View Article
mouseIF

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
View Article
mouseIF

Sci Adv

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Authors - Sefan Asamitsu
View Article
zebrafishIHC

Nat Commun

An injury-induced serotonergic neuron subpopulation contributes to axon regrowth and function restoration after spinal cord injury in zebrafish.

Authors - Chun-Xiao Huang
View Article
mouseIHC

Brain

Huntingtin silencing delays onset and slows progression of Huntington's disease: a biomarker study.

Authors - Hongshuai Liu
View Article
mouseIHC

Front Behav Neurosci

Lateral septal nucleus, dorsal part, and dentate gyrus are necessary for spatial and object recognition memory, respectively, in mice

Authors - Ying-Ke Jiang
View Article