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验证数据展示

  • WB analysis using 84401-4-RR

    WB analysis using 84401-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84401-4-RR

    WB analysis using 84401-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84401-4-RR (NeuN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 84401-4-RR

    IF Staining of mouse cerebellum using 84401-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 84401-4-RR

    IF Staining of mouse cerebellum using 84401-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (84401-4-RR, Clone: 241461G7 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84401-4-RR

    Affinity and Kinetic Characterization of 84401-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84401-4-RR against Human NeuN were performed. The affinity constant is 0.986 nM.

NeuN Recombinant antibody

NeuN Recombinant Antibody for WB, IF-P, ELISA
Cat No. 84401-4-RR

产品说明书

CloneNo. 241461G7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IF-P, ELISA

别名

Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3, Fox-1 homolog C

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, pig brain tissue, rat cerebellum tissue, mouse brain tissue, rat brain tissue
Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy NeuN Ready-To-Use IHC Kit. NeuN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84401-4-RR targets NeuN in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 NeuN fusion protein Ag25689 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hexaribonucleotide binding protein 3
别名 Neuronal nuclei antigen, NeuN antigen, HRNBP3, FOX3, Fox-1 homolog C
观测分子量 46-52 kDa
GenBank蛋白编号NM_001082575
基因名称 NeuN
Gene ID (NCBI) 146713
RRIDAB_3671930
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDA6NFN3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NeuN antibody 84401-4-RRDownload protocol
IF protocol for NeuN antibody 84401-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols